Sorghum lodging is an ongoing problem for sorghum producers and is often associated with Fusarium stalk rot. Recent studies have identified 7 Fusarium species associated with stalk rot in the major Australian sorghum-growing regions, with Fusarium thapsinum being the most prevalent. The use of Fusarium-resistant hybrids would be an important strategy in the integrated management of the disease, but the identification of resistant breeding lines is a time consuming process. Conventional resistance screening involves the inoculation of field-grown plants after flowering either by injection of a Fusarium spore suspension or by insertion of colonised toothpicks infected with Fusarium into the stem, followed by disease assessments several weeks later. However, a glasshouse screening technique conducted prior to flowering would have the advantage of obtaining quicker results. We have developed such a technique, which to date shows promise. Pot-grown sorghum plants (8th leaf stage) are injected with a 1 mL spore suspension of a Fusarium thapsinum isolate then slightly moisture stressed. After 21 days disease severity is assessed by splitting the stems longitudinally then counting the number of diseased internodes and measuring the length of the discoloured stem tissue. Our results have confirmed overseas reports that the line SC599 has a high level of resistance to Fusarium spp. compared to other lines (SC265 and IS8525). We are using the spore injection technique to quantify the resistance of 30 sorghum lines in glasshouse experiments and in field trials at two locations in southern Queensland to compare traditional resistance screening methods (inoculation post-flowering) with our method (inoculation during vegetative stage). This new technique has the potential to substantially reduce the time required for assessment of Fusarium resistance which will enable sorghum breeding programs to incorporate resistance into their breeding material more rapidly.